Asante Kotoko and rivals Hearts of Oak have been paired with Bekwai Youth Football Academy and Liberty Professionals respectively in the FA Cup round of 64.
In a draw held on Thursday, May 20 in the studios of Max TV will see regional rivals AshantiGold SC testing their strength against King Faisal.
Premiership sides Aduana Stars and Techiman Eleven Wonders will clash in the other regional derby, Berekum Chelsea and Bofoakwa Tano will be playing in the other regional derby.
Medeama will take on former Premier League campaigners Second Eleven Wise while Accra Great Olympics battle it out with Charity Stars FC.
Below is the FA Cup full draw:
Accra Great Olympics vs Charity Stars FC
Tema Youth FC vs Inter Allies FC
Accra City Stars FC vs Third World FC
Accra Hearts of Oak vs Liberty Professionals
Legon Cities vs Uncle T FC
Attram De Visser vs Mighty Jets FC
Okyeman Planners FC vs Young Wise FC
Kade United FC vs Okwawu United
Phar Rangers vs Dreams FC
Kintampo Top Talents FC vs Mighty Royals FC
Aduana Stars vs Eleven Wonders
Kato Freedom Fighters vs Bechem United
Kenyasi New Dreams FC vs Sunyani Reformers
Young Apostles FC vs TECHIMAN City/Unity FC
Berekum Chelsea vs Bofoakwa Tano FC
WAFA FC vs Akatsi Allstars FC
Hearts of Lions FC vs Bebeto FC
Wassaman FC vs Asokwa Deportivo
River Plate Athletic Club vs Thunderbolt FC
BYFA vs Asante Kotoko
King Faisal vs Ashantigold
Kintampo FC vs Bolga Soccer Masters
Real 24 hours FC vs Steadfast FC
Paga Crocodiles vs Sombo Freedom Stars
RTU vs Tamale City
Aboi Young Stars vs Samartex
Sekondi Eleven Wise vs Medeama SC
Sekondi Hasaacas vs Karela United FC